› 1emc

X-ray diffraction
2.3Å resolution

GREEN FLUORESCENT PROTEIN FROM AEQUOREA VICTORIA, MUTANT

Released:
DOI: 10.2210/pdb1emc/pdb
PDB entry 1emc coloured by chain, front view.
PDB entry 1emc coloured by chain, side view.
PDB entry 1emc coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
The structural basis for spectral variations in green fluorescent protein.
Palm GJ, Zdanov A, Gaitanaris GA, Stauber R, Pavlakis GN, Wlodawer A
Nat Struct Biol 4 361-5 (1997)
PMID: 9145105

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154560 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 237 amino acids
Theoretical weight: 26.94 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P42212 (Residues: 2-237; Coverage: 99%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand GYS 4 x GYS

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 93Å b: 52Å c: 103Å
α: 90° β: 101.8° γ: 90°
R-values:
R R work R free
0.205 0.205 0.288
Expression system: Escherichia coli BL21

Quick links

Citations

18 review citations

The green fluorescent protein.
Tsien RY. (1998)
17 more

1 mention without citation

Intracellular CXCR4⁺ cell targeting with T22-empowered protein-only nanoparticles.
Unzueta et al. (2012)