› 1en5

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE ANALYSIS OF THE E. COLI MANGANESE SUPEROXIDE DISMUTASE Y34F MUTANT

Released:
DOI: 10.2210/pdb1en5/pdb
PDB entry 1en5 coloured by chain, front view.
PDB entry 1en5 coloured by chain, side view.
PDB entry 1en5 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Outer sphere mutations perturb metal reactivity in manganese superoxide dismutase.
Edwards RA, Whittaker MM, Whittaker JW, Baker EN, Jameson GB
Biochemistry 40 15-27 (2001)
PMID: 11141052

Function and Biology Details

Reaction catalysed: 
2 superoxide + 2 H(+) = O(2) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132509 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Superoxide dismutase [Mn] Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 205 amino acids
Theoretical weight: 22.98 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P00448 (Residues: 2-206; Coverage: 100%)
Gene names: JW3879, b3908, sodA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MN 4 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2221
Unit cell:
a: 100.845Å b: 108.893Å c: 182.882Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.167 0.195
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Superoxide dismutases and superoxide reductases.
Sheng et al. (2014)
4 more

2 mentions without citation

Protein subunit interfaces: heterodimers versus homodimers.
Zhanhua et al. (2005)
1 more