› 1en7

X-ray diffraction
2.4Å resolution

ENDONUCLEASE VII (ENDOVII) FROM PHAGE T4

Released:
DOI: 10.2210/pdb1en7/pdb
PDB entry 1en7 coloured by chain, front view.
PDB entry 1en7 coloured by chain, side view.
PDB entry 1en7 coloured by chain, top view.
Source organism: Escherichia virus T4
Primary publication:
X-ray structure of T4 endonuclease VII: a DNA junction resolvase with a novel fold and unusual domain-swapped dimer architecture.
Raaijmakers H, Vix O, Törõ I, Golz S, Kemper B, Suck D
EMBO J 18 1447-58 (1999)
PMID: 10075917

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146548 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Recombination endonuclease VII Chains: A, B
Molecule details ›
Chains: A, B
Length: 157 amino acids
Theoretical weight: 18.17 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P13340 (Residues: 1-157; Coverage: 100%)
Gene name: 49
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS
Spacegroup: C2
Unit cell:
a: 144.99Å b: 39.45Å c: 75.75Å
α: 90° β: 106.2° γ: 90°
R-values:
R R work R free
0.24 0.24 0.308
Expression system: Escherichia coli BL21

Quick links

Citations

14 review citations

Bacteriophage T4 genome.
Miller et al. (2003)
13 more

13 mentions without citation

Holliday junction resolvases.
Wyatt et al. (2014)
12 more