› 1eqm

X-ray diffraction
1.5Å resolution

CRYSTAL STRUCTURE OF BINARY COMPLEX OF 6-HYDROXYMETHYL-7,8-DIHYDROPTERIN PYROPHOSPHOKINASE WITH ADENOSINE-5'-DIPHOSPHATE

Released:
DOI: 10.2210/pdb1eqm/pdb
PDB entry 1eqm coloured by chain, front view.
PDB entry 1eqm coloured by chain, side view.
PDB entry 1eqm coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Unusual conformational changes in 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase as revealed by X-ray crystallography and NMR.
Xiao B, Shi G, Gao J, Blaszczyk J, Liu Q, Ji X, Yan H
J Biol Chem 276 40274-81 (2001)
PMID: 11546767

Function and Biology Details

Reaction catalysed: 
ATP + 6-hydroxymethyl-7,8-dihydropterin = AMP + 6-hydroxymethyl-7,8-dihydropterin diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150686 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase Chain: A
Molecule details ›
Chain: A
Length: 158 amino acids
Theoretical weight: 17.97 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P26281 (Residues: 2-159; Coverage: 99%)
Gene names: JW0138, b0142, folK
Sequence domains: 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase (HPPK)
Structure domains: 7,8-Dihydro-6-hydroxymethylpterin-pyrophosphokinase HPPK

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand PO4 1 x PO4
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: C2
Unit cell:
a: 79.14Å b: 52.8Å c: 36.64Å
α: 90° β: 103.43° γ: 90°
R-values:
R R work R free
0.19 0.189 0.262
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

The folic acid biosynthesis pathway in bacteria: evaluation of potential for antibacterial drug discovery.
Bermingham et al. (2002)
3 more

2 mentions without citation

Role of protein conformational dynamics in the catalysis by 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase.
Yan et al. (2011)
1 more