› 1eug

X-ray diffraction
1.6Å resolution

CRYSTAL STRUCTURE OF ESCHERICHIA COLI URACIL DNA GLYCOSYLASE AND ITS COMPLEXES WITH URACIL AND GLYCEROL: STRUCTURE AND GLYCOSYLASE MECHANISM REVISITED

Released:
DOI: 10.2210/pdb1eug/pdb
PDB entry 1eug coloured by chain, front view.
PDB entry 1eug coloured by chain, side view.
PDB entry 1eug coloured by chain, top view.
Source organism: Escherichia coli B
Primary publication:
Crystal structure of Escherichia coli uracil DNA glycosylase and its complexes with uracil and glycerol: structure and glycosylase mechanism revisited.
Xiao G, Tordova M, Jagadeesh J, Drohat AC, Stivers JT, Gilliland GL
Proteins 35 13-24 (1999)
PMID: 10090282

Function and Biology Details

Reaction catalysed: 
Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146128 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uracil-DNA glycosylase Chain: A
Molecule details ›
Chain: A
Length: 229 amino acids
Theoretical weight: 25.71 KDa
Source organism: Escherichia coli B
Expression system: Escherichia coli
UniProt:
  • Canonical: P12295 (Residues: 1-229; Coverage: 100%)
Gene names: JW2564, b2580, ung
Sequence domains: Uracil DNA glycosylase superfamily
Structure domains: Uracil-DNA glycosylase-like domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER
Spacegroup: P212121
Unit cell:
a: 55.13Å b: 61.32Å c: 64.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 not available 0.25
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Uracil in DNA--occurrence, consequences and repair.
Krokan et al. (2002)
8 more

5 mentions without citation

Recent advances in the structural mechanisms of DNA glycosylases.
Brooks et al. (2013)
4 more