› 1eut

X-ray diffraction
2.5Å resolution

SIALIDASE, LARGE 68KD FORM, COMPLEXED WITH GALACTOSE

Released:
DOI: 10.2210/pdb1eut/pdb
PDB entry 1eut coloured by chain, front view.
PDB entry 1eut coloured by chain, side view.
PDB entry 1eut coloured by chain, top view.
Source organism: Micromonospora viridifaciens
Primary publication:
The three domains of a bacterial sialidase: a beta-propeller, an immunoglobulin module and a galactose-binding jelly-roll.
Gaskell A, Crennell S, Taylor G
Structure 3 1197-205 (1995)
PMID: 8591030

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169775 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sialidase Chain: A
Molecule details ›
Chain: A
Length: 605 amino acids
Theoretical weight: 64.65 KDa
Source organism: Micromonospora viridifaciens
UniProt:
  • Canonical: Q02834 (Residues: 43-647; Coverage: 99%)
Gene name: nedA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P21
Unit cell:
a: 51.28Å b: 117.3Å c: 60.24Å
α: 90° β: 96.17° γ: 90°
R-values:
R R work R free
0.214 0.214 not available

Quick links

Citations

18 review citations

Carbohydrate-binding modules: fine-tuning polysaccharide recognition.
Boraston et al. (2004)
17 more

10 mentions without citation

Unraveling virus relationships by structure-based phylogenetic classification.
Ng et al. (2020)
9 more