› 1ezf

X-ray diffraction
2.15Å resolution

CRYSTAL STRUCTURE OF HUMAN SQUALENE SYNTHASE

Released:
DOI: 10.2210/pdb1ezf/pdb
PDB entry 1ezf coloured by chain, front view.
PDB entry 1ezf coloured by chain, side view.
PDB entry 1ezf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human squalene synthase. A key enzyme in cholesterol biosynthesis.
Pandit J, Danley DE, Schulte GK, Mazzalupo S, Pauly TA, Hayward CM, Hamanaka ES, Thompson JF, Harwood HJ
J Biol Chem 275 30610-7 (2000)
PMID: 10896663

Function and Biology Details

Reaction catalysed: 
(1a) 2 farnesyl diphosphate = diphosphate + presqualene diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153385 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Squalene synthase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 340 amino acids
Theoretical weight: 39.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P37268 (Residues: 31-370; Coverage: 82%)
Gene name: FDFT1
Sequence domains: Squalene/phytoene synthase
Structure domains: Farnesyl Diphosphate Synthase

Ligands and Environments

1 bound ligand:
Ligand IN0 3 x IN0
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 76.76Å b: 87.92Å c: 154.17Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.213 0.262
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Structural and Chemical Biology of Terpenoid Cyclases.
Christianson DW. (2017)
12 more

23 mentions without citation

Terpenoid synthase structures: a so far incomplete view of complex catalysis.
Gao et al. (2012)
22 more