› 1ezg

X-ray diffraction
1.4Å resolution

CRYSTAL STRUCTURE OF ANTIFREEZE PROTEIN FROM THE BEETLE, TENEBRIO MOLITOR

Released:
DOI: 10.2210/pdb1ezg/pdb
PDB entry 1ezg coloured by chain, front view.
PDB entry 1ezg coloured by chain, side view.
PDB entry 1ezg coloured by chain, top view.
Source organism: Tenebrio molitor
Primary publication:
Mimicry of ice structure by surface hydroxyls and water of a beta-helix antifreeze protein.
Liou YC, Tocilj A, Davies PL, Jia Z
Nature 406 322-4 (2000)
PMID: 10917536

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127562 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thermal hysteresis protein YL-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 84 amino acids
Theoretical weight: 8.39 KDa
Source organism: Tenebrio molitor
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O16119 (Residues: 29-112; Coverage: 100%)
Sequence domains: Insect antifreeze protein repeat
Structure domains: Insect antifreeze protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P65
Unit cell:
a: 73.832Å b: 73.832Å c: 53.13Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.16 0.16 0.2
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

34 review citations

Molecular biomimetics: nanotechnology through biology.
Sarikaya et al. (2003)
33 more

24 mentions without citation

Chemistry and Enzymology of Disulfide Cross-Linking in Proteins.
Fass et al. (2018)
23 more