› 1f09

X-ray diffraction
2.14Å resolution

CRYSTAL STRUCTURE OF THE GREEN FLUORESCENT PROTEIN (GFP) VARIANT YFP-H148Q WITH TWO BOUND IODIDES

Released:
DOI: 10.2210/pdb1f09/pdb
PDB entry 1f09 coloured by chain, front view.
PDB entry 1f09 coloured by chain, side view.
PDB entry 1f09 coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Crystallographic and energetic analysis of binding of selected anions to the yellow variants of green fluorescent protein.
Wachter RM, Yarbrough D, Kallio K, Remington SJ
J Mol Biol 301 157-71 (2000)
PMID: 10926499

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 26.95 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand IOD 2 x IOD
1 modified residue:
Ligand CR2 1 x CR2

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 51.197Å b: 62.818Å c: 68.667Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.204 not available
Expression system: Escherichia coli

Quick links

Citations

21 review citations

Creating new fluorescent probes for cell biology.
Zhang et al. (2002)
20 more

9 mentions without citation

Twenty years of fluorescence imaging of intracellular chloride.
Arosio et al. (2014)
8 more