1f36

X-ray diffraction
2.65Å resolution

THE CRYSTAL STRUCTURE OF FIS MUTANT K36E REVEALS THAT THE TRANSACTIVATION REGION OF THE FIS PROTEIN CONTAINS EXTENDED MOBILE BETA-HAIRPIN ARMS

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-141419 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-binding protein Fis Chains: A, B
Molecule details ›
Chains: A, B
Length: 98 amino acids
Theoretical weight: 11.25 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6R3 (Residues: 1-98; Coverage: 100%)
Gene names: JW3229, b3261, fis
Sequence domains: Bacterial regulatory protein, Fis family
Structure domains: Homeodomain-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P6522
Unit cell:
a: 51.9Å b: 51.9Å c: 294.93Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.222 0.222 0.277
Expression system: Escherichia coli