› 1f5j

X-ray diffraction
1.8Å resolution

CRYSTAL STRUCTURE OF XYNB, A HIGHLY THERMOSTABLE BETA-1,4-XYLANASE FROM DICTYOGLOMUS THERMOPHILUM RT46B.1, AT 1.8 A RESOLUTION

Released:
DOI: 10.2210/pdb1f5j/pdb
PDB entry 1f5j coloured by chain, front view.
PDB entry 1f5j coloured by chain, side view.
PDB entry 1f5j coloured by chain, top view.
Source organism: Dictyoglomus thermophilum
Primary publication:
Structure of XynB, a highly thermostable beta-1,4-xylanase from Dictyoglomus thermophilum Rt46B.1, at 1.8 A resolution.
McCarthy AA, Morris DD, Bergquist PL, Baker EN
Acta Crystallogr D Biol Crystallogr 56 1367-75 (2000)
PMID: 11053833

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160208 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endo-1,4-beta-xylanase Chains: A, B
Molecule details ›
Chains: A, B
Length: 199 amino acids
Theoretical weight: 22.31 KDa
Source organism: Dictyoglomus thermophilum
Expression system: Escherichia coli
UniProt:
  • Canonical: P77853 (Residues: 29-227; Coverage: 59%)
Gene name: xynB
Sequence domains: Glycosyl hydrolases family 11
Structure domains: Jelly Rolls

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P43
Unit cell:
a: 91.29Å b: 91.29Å c: 44.93Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.185 0.222
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Xylanases, xylanase families and extremophilic xylanases.
Collins et al. (2005)
3 more

7 mentions without citation

Bacterial xylanases: biology to biotechnology.
Chakdar et al. (2016)
6 more