› 1f8r

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF L-AMINO ACID OXIDASE FROM CALLOSELASMA RHODOSTOMA COMPLEXED WITH CITRATE

Released:
DOI: 10.2210/pdb1f8r/pdb
PDB entry 1f8r coloured by chain, front view.
PDB entry 1f8r coloured by chain, side view.
PDB entry 1f8r coloured by chain, top view.
Source organism: Calloselasma rhodostoma
Primary publication:
The structure of L-amino acid oxidase reveals the substrate trajectory into an enantiomerically conserved active site.
Pawelek PD, Cheah J, Coulombe R, Macheroux P, Ghisla S, Vrielink A
EMBO J 19 4204-15 (2000)
PMID: 10944103

Function and Biology Details

Reaction catalysed: 
An L-amino acid + H(2)O + O(2) = a 2-oxo carboxylate + NH(3) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160512 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
L-amino-acid oxidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 498 amino acids
Theoretical weight: 56.3 KDa
Source organism: Calloselasma rhodostoma
UniProt:
  • Canonical: P81382 (Residues: 19-516; Coverage: 100%)
Sequence domains: Flavin containing amine oxidoreductase
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 4 x FAD
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
2 bound ligands:
Ligand NAG 4 x NAG
Ligand CIT 4 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P21
Unit cell:
a: 79.496Å b: 154.962Å c: 102.947Å
α: 90° β: 109.49° γ: 90°
R-values:
R R work R free
0.185 0.185 0.21

Quick links

Citations

24 review citations

Snake venom L-amino acid oxidases.
Du et al. (2002)
23 more

12 mentions without citation

Structure-Function Studies and Mechanism of Action of Snake Venom L-Amino Acid Oxidases.
Ullah A. (2020)
11 more