› 1f9a

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE ANALYSIS OF NMN ADENYLYLTRANSFERASE FROM METHANOCOCCUS JANNASCHII

Released:
DOI: 10.2210/pdb1f9a/pdb
PDB entry 1f9a coloured by chain, front view.
PDB entry 1f9a coloured by chain, side view.
PDB entry 1f9a coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Primary publication:
Structure of nicotinamide mononucleotide adenylyltransferase: a key enzyme in NAD(+) biosynthesis.
D'Angelo I, Raffaelli N, Dabusti V, Lorenzi T, Magni G, Rizzi M
Structure 8 993-1004 (2000)
PMID: 10986466

Function and Biology Details

Reaction catalysed: 
ATP + nicotinamide ribonucleotide = diphosphate + NAD(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176504 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nicotinamide-nucleotide adenylyltransferase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 168 amino acids
Theoretical weight: 19.62 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q57961 (Residues: 1-168; Coverage: 100%)
Gene name: MJ0541
Sequence domains: Cytidylyltransferase-like
Structure domains: HUPs

Ligands and Environments

2 bound ligands:
Ligand MG 6 x MG
Ligand ATP 6 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P21
Unit cell:
a: 78.768Å b: 112.642Å c: 79.869Å
α: 90° β: 116.94° γ: 90°
R-values:
R R work R free
0.215 0.215 0.264
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Determination of protein function, evolution and interactions by structural genomics.
Teichmann et al. (2001)
1 more

7 mentions without citation

Recognition of functional sites in protein structures.
Shulman-Peleg et al. (2004)
6 more