› 1f9c

X-ray diffraction
2.5Å resolution

CRYSTAL STRUCTURE OF MLE D178N VARIANT

Released:
DOI: 10.2210/pdb1f9c/pdb
PDB entry 1f9c coloured by chain, front view.
PDB entry 1f9c coloured by chain, side view.
PDB entry 1f9c coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
Buried charged surface in proteins.
Kajander T, Kahn PC, Passila SH, Cohen DC, Lehtiö L, Adolfsen W, Warwicker J, Schell U, Goldman A
Structure 8 1203-14 (2000)
PMID: 11080642

Function and Biology Details

Reaction catalysed: 
(+)-muconolactone = cis,cis-muconate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175993 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mandelate racemase/muconate lactonizing enzyme C-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 372 amino acids
Theoretical weight: 40.11 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: Q51958 (Residues: 2-373; Coverage: 100%)
Gene name: catB
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MN 2 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X31
Spacegroup: I4
Unit cell:
a: 136.979Å b: 136.979Å c: 82.968Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.187 0.234
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Protein structure, stability and solubility in water and other solvents.
Pace et al. (2004)
4 more