› 1fa0

X-ray diffraction
2.6Å resolution

STRUCTURE OF YEAST POLY(A) POLYMERASE BOUND TO MANGANATE AND 3'-DATP

Released:
DOI: 10.2210/pdb1fa0/pdb
PDB entry 1fa0 coloured by chain, front view.
PDB entry 1fa0 coloured by chain, side view.
PDB entry 1fa0 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure of yeast poly(A) polymerase alone and in complex with 3'-dATP.
Bard J, Zhelkovsky AM, Helmling S, Earnest TN, Moore CL, Bohm A
Science 289 1346-9 (2000)
PMID: 10958780

Function and Biology Details

Reaction catalysed: 
ATP + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151506 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly(A) polymerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 537 amino acids
Theoretical weight: 61.44 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P29468 (Residues: 1-537; Coverage: 95%)
Gene names: PAP1, YKR002W
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand MN 4 x MN
Ligand 3AT 2 x 3AT
Ligand 3AD 2 x 3AD
Ligand POP 1 x POP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 73.8Å b: 109.1Å c: 238.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.233 0.278
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Protein factors in pre-mRNA 3'-end processing.
Mandel et al. (2008)
14 more

14 mentions without citation

Delineating the structural blueprint of the pre-mRNA 3'-end processing machinery.
Xiang et al. (2014)
13 more