› 1flt

X-ray diffraction
1.7Å resolution

VEGF IN COMPLEX WITH DOMAIN 2 OF THE FLT-1 RECEPTOR

Released:
DOI: 10.2210/pdb1flt/pdb
PDB entry 1flt coloured by chain, front view.
PDB entry 1flt coloured by chain, side view.
PDB entry 1flt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure at 1.7 A resolution of VEGF in complex with domain 2 of the Flt-1 receptor.
Wiesmann C, Fuh G, Christinger HW, Eigenbrot C, Wells JA, de Vos AM
Cell 91 695-704 (1997)
PMID: 9393862

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
hetero octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-147430 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
N-VEGF Chains: V, W
Molecule details ›
Chains: V, W
Length: 98 amino acids
Theoretical weight: 11.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P15692 (Residues: 218-315; Coverage: 25%)
Gene names: VEGF, VEGFA
Structure domains: Cystine-knot cytokines
Vascular endothelial growth factor receptor 1 Chains: X, Y
Molecule details ›
Chains: X, Y
Length: 95 amino acids
Theoretical weight: 10.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P17948 (Residues: 132-226; Coverage: 7%)
Gene names: FLT, FLT1, FRT, VEGFR1
Sequence domains: Vascular endothelial growth factor receptor 1-like, Ig-like domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: C2
Unit cell:
a: 81.44Å b: 71.13Å c: 77.86Å
α: 90° β: 105.28° γ: 90°
R-values:
R R work R free
0.198 0.198 0.261
Expression system: Escherichia coli

Quick links

Citations

67 review citations

Cell signaling by receptor tyrosine kinases.
Schlessinger J. (2000)
66 more

50 mentions without citation

Tying the knot: the cystine signature and molecular-recognition processes of the vascular endothelial growth factor family of angiogenic cytokines.
Iyer et al. (2011)
49 more