› 1fmf

Solution NMR

REFINED SOLUTION STRUCTURE OF THE (13C,15N-LABELED) B12-BINDING SUBUNIT OF GLUTAMATE MUTASE FROM CLOSTRIDIUM TETANOMORPHUM

Released:
DOI: 10.2210/pdb1fmf/pdb
PDB entry 1fmf coloured by chain, ensemble of 30 models, front view.
PDB entry 1fmf coloured by chain, ensemble of 30 models, side view.
PDB entry 1fmf coloured by chain, ensemble of 30 models, top view.
Source organism: Clostridium tetanomorphum
Primary publication:
A protein pre-organized to trap the nucleotide moiety of coenzyme B(12): refined solution structure of the B(12)-binding subunit of glutamate mutase from Clostridium tetanomorphum.
Hoffmann B, Tollinger M, Konrat R, Huhta M, Marsh EN, Kräutler B
Chembiochem 2 643-55 (2001)
PMID: 11828501

Function and Biology Details

Reaction catalysed: 
L-threo-3-methylaspartate = L-glutamate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170208 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate mutase sigma subunit Chain: A
Molecule details ›
Chain: A
Length: 137 amino acids
Theoretical weight: 14.76 KDa
Source organism: Clostridium tetanomorphum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q05488 (Residues: 1-137; Coverage: 100%)
Gene names: glmS, mutS
Sequence domains: B12 binding domain
Structure domains: Cobalamin-binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: distance geometry, simulated annealing, simulated annealing refinement, energy minimization
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Coenzyme B(12) dependent glutamate mutase.
Gruber et al. (2002)
1 other article

1 mention without citation

Stretching to understand proteins - a survey of the protein data bank.
Sułkowska et al. (2008)