› 1fmi

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF HUMAN CLASS I ALPHA1,2-MANNOSIDASE

Released:
DOI: 10.2210/pdb1fmi/pdb
PDB entry 1fmi coloured by chain, front view.
PDB entry 1fmi coloured by chain, side view.
PDB entry 1fmi coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for catalysis and inhibition of N-glycan processing class I alpha 1,2-mannosidases.
Vallee F, Karaveg K, Herscovics A, Moremen KW, Howell PL
J Biol Chem 275 41287-98 (2000)
PMID: 10995765

Function and Biology Details

Reaction catalysed: 
(1a) Man(9)GlcNAc(2)-[protein] + H(2)O = Man(8)GlcNAc(2)-[protein] (isomer 8A(1,2,3)B(1,2)) + beta-D-mannopyranose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193855 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase Chain: A
Molecule details ›
Chain: A
Length: 458 amino acids
Theoretical weight: 52.58 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q9UKM7 (Residues: 242-697; Coverage: 65%)
Gene names: MAN1B1, UNQ747/PRO1477
Sequence domains: Glycosyl hydrolase family 47
Structure domains: Glycosyltransferase

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P3121
Unit cell:
a: 95.829Å b: 95.829Å c: 136.853Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.222 0.222 0.25
Expression system: Saccharomyces cerevisiae

Quick links

Citations

16 review citations

Glycoside hydrolases and glycosyltransferases: families and functional modules.
Bourne et al. (2001)
15 more

1 mention without citation

ER-resident protein 46 (ERp46) triggers the mannose-trimming activity of ER degradation-enhancing α-mannosidase-like protein 3 (EDEM3).
Yu et al. (2018)