› 1fq9

X-ray diffraction
3Å resolution

CRYSTAL STRUCTURE OF A TERNARY FGF2-FGFR1-HEPARIN COMPLEX

Released:
DOI: 10.2210/pdb1fq9/pdb
PDB entry 1fq9 coloured by chain, front view.
PDB entry 1fq9 coloured by chain, side view.
PDB entry 1fq9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of a ternary FGF-FGFR-heparin complex reveals a dual role for heparin in FGFR binding and dimerization.
Schlessinger J, Plotnikov AN, Ibrahimi OA, Eliseenkova AV, Yeh BK, Yayon A, Linhardt RJ, Mohammadi M
Mol Cell 6 743-50 (2000)
PMID: 11030354

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140537 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Fibroblast growth factor 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 132 amino acids
Theoretical weight: 15.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09038 (Residues: 157-288; Coverage: 46%)
Gene names: FGF2, FGFB
Sequence domains: Fibroblast growth factor
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)
Fibroblast growth factor receptor 1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 225 amino acids
Theoretical weight: 25.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P11362 (Residues: 141-365; Coverage: 28%)
Gene names: BFGFR, CEK, FGFBR, FGFR1, FLG, FLT2, HBGFR
Sequence domains: Immunoglobulin I-set domain
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: SGN, IDU, IDS, UAP
Ligand SGN 4 x SGN
Ligand IDU 1 x IDU
Ligand IDS 2 x IDS
Ligand UAP 1 x UAP
Carbohydrate polymer : NEW Components: SGN, IDS, UAP
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P41212
Unit cell:
a: 98.888Å b: 98.888Å c: 196.292Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.229 0.282
Expression system: Escherichia coli

Quick links

Citations

182 review citations

Cell signaling by receptor tyrosine kinases.
Schlessinger J. (2000)
181 more

73 mentions without citation

Demystifying heparan sulfate-protein interactions.
Xu et al. (2014)
72 more