› 1fr2

X-ray diffraction
1.6Å resolution

CRYSTAL STRUCTURE OF THE E9 DNASE DOMAIN WITH A MUTANT IMMUNITY PROTEIN IM9(E41A)

Released:
DOI: 10.2210/pdb1fr2/pdb
PDB entry 1fr2 coloured by chain, front view.
PDB entry 1fr2 coloured by chain, side view.
PDB entry 1fr2 coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Kuhlmann UC, Pommer AJ, Moore GM, James R, Kleanthous C, Hemmings AM

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140796 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Colicin-E9 immunity protein Chain: A
Molecule details ›
Chain: A
Length: 86 amino acids
Theoretical weight: 9.53 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P13479 (Residues: 1-86; Coverage: 100%)
Gene names: ceiE9, imm
Sequence domains: Colicin immunity protein / pyocin immunity protein
Structure domains: Colicin E immunity protein
Colicin-E9 Chain: B
Molecule details ›
Chain: B
Length: 134 amino acids
Theoretical weight: 15.12 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P09883 (Residues: 450-582; Coverage: 23%)
Gene names: cei, col
Sequence domains: Colicin/Pyocin-S2, DNase domain
Structure domains: Colicin/pyocin, DNase domain

Ligands and Environments

2 bound ligands:
Ligand PO4 1 x PO4
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 45.05Å b: 51.64Å c: 87.12Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.205 0.232
Expression system: Escherichia coli

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