› 1fwk

X-ray diffraction
2.1Å resolution

CRYSTAL STRUCTURE OF HOMOSERINE KINASE COMPLEXED WITH ADP

Released:
DOI: 10.2210/pdb1fwk/pdb
PDB entry 1fwk coloured by chain, front view.
PDB entry 1fwk coloured by chain, side view.
PDB entry 1fwk coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Primary publication:
Structure and mechanism of homoserine kinase: prototype for the GHMP kinase superfamily.
Zhou T, Daugherty M, Grishin NV, Osterman AL, Zhang H
Structure 8 1247-57 (2000)
PMID: 11188689

Function and Biology Details

Reaction catalysed: 
ATP + L-homoserine = ADP + O-phospho-L-homoserine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-176635 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Homoserine kinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 296 amino acids
Theoretical weight: 32.3 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q58504 (Residues: 1-296; Coverage: 100%)
Gene names: MJ1104, thrB
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ADP 4 x ADP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P21
Unit cell:
a: 62.206Å b: 129.147Å c: 109.773Å
α: 90° β: 105.52° γ: 90°
R-values:
R R work R free
0.209 0.209 0.246
Expression system: Escherichia coli

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Citations

12 review citations

Structure and function of enzymes of the Leloir pathway for galactose metabolism.
Holden et al. (2003)
11 more

7 mentions without citation

Design principles underpinning the regulatory diversity of protein kinases.
Oruganty et al. (2012)
6 more