› 1g24

X-ray diffraction
1.7Å resolution

THE CRYSTAL STRUCTURE OF EXOENZYME C3 FROM CLOSTRIDIUM BOTULINUM

Released:
DOI: 10.2210/pdb1g24/pdb
PDB entry 1g24 coloured by chain, front view.
PDB entry 1g24 coloured by chain, side view.
PDB entry 1g24 coloured by chain, top view.
Source organism: Clostridium botulinum
Primary publication:
Crystal structure and novel recognition motif of rho ADP-ribosylating C3 exoenzyme from Clostridium botulinum: structural insights for recognition specificity and catalysis.
Han S, Arvai AS, Clancy SB, Tainer JA
J Mol Biol 305 95-107 (2001)
PMID: 11114250

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147514 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mono-ADP-ribosyltransferase C3 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 211 amino acids
Theoretical weight: 23.59 KDa
Source organism: Clostridium botulinum
Expression system: Escherichia coli
UniProt:
  • Canonical: P15879 (Residues: 41-251; Coverage: 100%)
Gene name: C3
Sequence domains: ADP-ribosyltransferase exoenzyme
Structure domains: Toxin ADP-ribosyltransferase; Chain A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: C2
Unit cell:
a: 233.747Å b: 73.757Å c: 73.749Å
α: 90° β: 107° γ: 90°
R-values:
R R work R free
0.24 0.24 0.265
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Toward a unified nomenclature for mammalian ADP-ribosyltransferases.
Hottiger et al. (2010)
18 more

8 mentions without citation

Novel bacterial ADP-ribosylating toxins: structure and function.
Simon et al. (2014)
7 more