› 1g2f

X-ray diffraction
2Å resolution

STRUCTURE OF A CYS2HIS2 ZINC FINGER/TATA BOX COMPLEX (TATAZF;CLONE #6)

Released:
DOI: 10.2210/pdb1g2f/pdb
PDB entry 1g2f coloured by chain, front view.
PDB entry 1g2f coloured by chain, side view.
PDB entry 1g2f coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Beyond the "recognition code": structures of two Cys2His2 zinc finger/TATA box complexes.
Wolfe SA, Grant RA, Elrod-Erickson M, Pabo CO
Structure 9 717-23 (2001)
PMID: 11587646

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113840 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Early growth response protein 1 Chains: C, F
Molecule details ›
Chains: C, F
Length: 90 amino acids
Theoretical weight: 10.63 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08046 (Residues: 333-421; Coverage: 17%)
Gene names: Egr-1, Egr1, Krox-24
Sequence domains: Zinc finger, C2H2 type
Structure domains: Classic Zinc Finger
5'-D(*GP*AP*CP*GP*CP*TP*AP*TP*AP*AP*AP*AP*GP*GP*AP*G)-3' Chains: A, D
Molecule details ›
Chains: A, D
Length: 16 nucleotides
Theoretical weight: 4.98 KDa
Source organism: Mus musculus
Expression system: Not provided
5'-D(*TP*CP*CP*TP*TP*TP*TP*AP*TP*AP*GP*CP*GP*TP*CP*C)-3' Chains: B, E
Molecule details ›
Chains: B, E
Length: 16 nucleotides
Theoretical weight: 4.82 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 6 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P64
Unit cell:
a: 104.8Å b: 104.8Å c: 104.2Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.233 0.233 0.263
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

10 review citations

Genome editing with engineered zinc finger nucleases.
Urnov et al. (2010)
9 more

11 mentions without citation

DNA conformations and their sequence preferences.
Svozil et al. (2008)
10 more