› 1g4c

X-ray diffraction
1.65Å resolution

CRYSTAL STRUCTURE OF A COMPLEX OF HPPK(R92A) FROM E.COLI WITH MG2+ AT 1.65 ANGSTROM RESOLUTION

Released:
DOI: 10.2210/pdb1g4c/pdb
PDB entry 1g4c coloured by chain, front view.
PDB entry 1g4c coloured by chain, side view.
PDB entry 1g4c coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Dynamic roles of arginine residues 82 and 92 of Escherichia coli 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase: crystallographic studies.
Blaszczyk J, Li Y, Shi G, Yan H, Ji X
Biochemistry 42 1573-80 (2003)
PMID: 12578370

Function and Biology Details

Reaction catalysed: 
ATP + 6-hydroxymethyl-7,8-dihydropterin = AMP + 6-hydroxymethyl-7,8-dihydropterin diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150686 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 158 amino acids
Theoretical weight: 17.88 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P26281 (Residues: 2-159; Coverage: 99%)
Gene names: JW0138, b0142, folK
Sequence domains: 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase (HPPK)
Structure domains: 7,8-Dihydro-6-hydroxymethylpterin-pyrophosphokinase HPPK

Ligands and Environments

2 bound ligands:
Ligand MG 3 x MG
Ligand CL 3 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: P21
Unit cell:
a: 42.351Å b: 47.21Å c: 71.306Å
α: 90° β: 105.61° γ: 90°
R-values:
R R work R free
0.205 0.172 0.236
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Folate biosynthesis pathway: mechanisms and insights into drug design for infectious diseases.
Bertacine Dias et al. (2018)
1 more