› 1g60

X-ray diffraction
1.74Å resolution

Crystal Structure of Methyltransferase MboIIa (Moraxella bovis)

Released:
DOI: 10.2210/pdb1g60/pdb
PDB entry 1g60 coloured by chain, front view.
PDB entry 1g60 coloured by chain, side view.
PDB entry 1g60 coloured by chain, top view.
Source organism: Moraxella bovis
Primary publication:
Crystal structure of MboIIA methyltransferase.
Osipiuk J, Walsh MA, Joachimiak A
Nucleic Acids Res 31 5440-8 (2003)
PMID: 12954781

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149889 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type II methyltransferase M1.MboII Chains: A, B
Molecule details ›
Chains: A, B
Length: 260 amino acids
Theoretical weight: 30.19 KDa
Source organism: Moraxella bovis
Expression system: Escherichia coli
UniProt:
  • Canonical: P23192 (Residues: 1-260; Coverage: 100%)
Gene name: mboIIM
Sequence domains: DNA methylase
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments


Cofactor: Ligand SAM 2 x SAM
1 bound ligand:
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 60.818Å b: 98.699Å c: 50.894Å
α: 90° β: 101.93° γ: 90°
R-values:
R R work R free
0.199 0.198 0.221
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Readers, writers and erasers of N6-methylated adenosine modification.
Wu et al. (2017)
4 more

11 mentions without citation

Many paths to methyltransfer: a chronicle of convergence.
Schubert et al. (2003)
10 more