› 1g6y

X-ray diffraction
2.8Å resolution

CRYSTAL STRUCTURE OF THE GLOBULAR REGION OF THE PRION PROTEIN URE2 FROM YEAST SACCHAROMYCES CEREVISIAE

Released:
DOI: 10.2210/pdb1g6y/pdb
PDB entry 1g6y coloured by chain, front view.
PDB entry 1g6y coloured by chain, side view.
PDB entry 1g6y coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure of the globular region of the prion protein Ure2 from the yeast Saccharomyces cerevisiae.
Bousset L, Belrhali H, Janin J, Melki R, Morera S
Structure 9 39-46 (2001)
PMID: 11342133

Function and Biology Details

Reaction catalysed: 
2 glutathione + H(2)O(2) = glutathione disulfide + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149892 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcriptional regulator URE2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 261 amino acids
Theoretical weight: 29.97 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P23202 (Residues: 94-354; Coverage: 74%)
Gene names: N1165, URE2, YNL229C
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 54.49Å b: 73.7Å c: 130.97Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.218 0.293
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Prions in yeast.
Liebman et al. (2012)
9 more

2 mentions without citation

Yeast prions assembly and propagation: contributions of the prion and non-prion moieties and the nature of assemblies.
Kabani et al. (2011)
1 more