› 1g7y

X-ray diffraction
2.5Å resolution

THE CRYSTAL STRUCTURE OF THE 58KD VEGETATIVE LECTIN FROM THE TROPICAL LEGUME DOLICHOS BIFLORUS

Released:
DOI: 10.2210/pdb1g7y/pdb
PDB entry 1g7y coloured by chain, front view.
PDB entry 1g7y coloured by chain, side view.
PDB entry 1g7y coloured by chain, top view.
Source organism: Vigna unguiculata subsp. cylindrica
Primary publication:
Weak protein-protein interactions in lectins: the crystal structure of a vegetative lectin from the legume Dolichos biflorus.
Buts L, Dao-Thi MH, Loris R, Wyns L, Etzler M, Hamelryck T
J Mol Biol 309 193-201 (2001)
PMID: 11491289

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148652 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Lectin DB58 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 253 amino acids
Theoretical weight: 27.15 KDa
Source organism: Vigna unguiculata subsp. cylindrica
UniProt:
  • Canonical: P19588 (Residues: 23-275; Coverage: 100%)
Sequence domains: Legume lectin domain
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUL
Carbohydrate polymer
2 bound ligands:
Ligand CA 6 x CA
Ligand MN 6 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P212121
Unit cell:
a: 100.941Å b: 130.272Å c: 137.124Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.221 0.247

Quick links

Citations

3 review citations

N-linked oligosaccharides as outfitters for glycoprotein folding, form and function.
Mitra et al. (2006)
2 more

3 mentions without citation

Research advances and prospects of legume lectins.
Katoch et al. (2021)
2 more