› 1g8f

X-ray diffraction
1.95Å resolution

ATP SULFURYLASE FROM S. CEREVISIAE

Released:
DOI: 10.2210/pdb1g8f/pdb
PDB entry 1g8f coloured by chain, front view.
PDB entry 1g8f coloured by chain, side view.
PDB entry 1g8f coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structure of ATP sulfurylase from Saccharomyces cerevisiae, a key enzyme in sulfate activation.
Ullrich TC, Blaesse M, Huber R
EMBO J 20 316-29 (2001)
PMID: 11157739

Function and Biology Details

Reaction catalysed: 
ATP + sulfate = diphosphate + adenylyl sulfate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140165 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sulfate adenylyltransferase Chain: A
Molecule details ›
Chain: A
Length: 511 amino acids
Theoretical weight: 57.82 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P08536 (Residues: 1-511; Coverage: 100%)
Gene names: J1436, MET3, YJR010W
Sequence domains:
Structure domains:

Ligands and Environments

7 bound ligands:
Ligand CD 5 x CD
Ligand CA 3 x CA
Ligand NA 2 x NA
Ligand MG 1 x MG
Ligand SO4 2 x SO4
Ligand TRS 1 x TRS
Ligand ACY 11 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: R32
Unit cell:
a: 187.094Å b: 187.094Å c: 115.999Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.196 0.231

Quick links

Citations

9 review citations

Sulfur assimilation and glutathione metabolism under cadmium stress in yeast, protists and plants.
Mendoza-Cózatl et al. (2005)
8 more

7 mentions without citation

Adenosine-5'-phosphosulfate--a multifaceted modulator of bifunctional 3'-phospho-adenosine-5'-phosphosulfate synthases and related enzymes.
Mueller et al. (2013)
6 more