› 1g8y

X-ray diffraction
2.4Å resolution

CRYSTAL STRUCTURE OF THE HEXAMERIC REPLICATIVE HELICASE REPA OF PLASMID RSF1010

Released:
DOI: 10.2210/pdb1g8y/pdb
PDB entry 1g8y coloured by chain, front view.
PDB entry 1g8y coloured by chain, side view.
PDB entry 1g8y coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of the hexameric replicative helicase RepA of plasmid RSF1010.
Niedenzu T, Röleke D, Bains G, Scherzinger E, Saenger W
J Mol Biol 306 479-87 (2001)
PMID: 11178907

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148960 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Regulatory protein RepA Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 279 amino acids
Theoretical weight: 29.95 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P20356 (Residues: 1-279; Coverage: 100%)
Gene name: repA
Sequence domains: AAA domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P21
Unit cell:
a: 104.432Å b: 179.156Å c: 116.289Å
α: 90° β: 108.8° γ: 90°
R-values:
R R work R free
0.221 0.221 0.264
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Structure and mechanism of helicases and nucleic acid translocases.
Singleton et al. (2007)
16 more

8 mentions without citation

Common mechanisms of DNA translocation motors in bacteria and viruses using one-way revolution mechanism without rotation.
Guo et al. (2014)
7 more