› 1g9f

X-ray diffraction
2.5Å resolution

CRYSTAL STRUCTURE OF THE SOYBEAN AGGLUTININ IN A COMPLEX WITH A BIANTENNARY BLOOD GROUP ANTIGEN ANALOG

Released:
DOI: 10.2210/pdb1g9f/pdb
PDB entry 1g9f coloured by chain, front view.
PDB entry 1g9f coloured by chain, side view.
PDB entry 1g9f coloured by chain, top view.
Source organism: Glycine max
Primary publication:
Weak protein-protein interactions in lectins: the crystal structure of a vegetative lectin from the legume Dolichos biflorus.
Buts L, Dao-Thi MH, Loris R, Wyns L, Etzler M, Hamelryck T
J Mol Biol 309 193-201 (2001)
PMID: 11491289

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138339 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Lectin Chain: A
Molecule details ›
Chain: A
Length: 253 amino acids
Theoretical weight: 27.6 KDa
Source organism: Glycine max
UniProt:
  • Canonical: P05046 (Residues: 33-285; Coverage: 100%)
Gene name: LE1
Sequence domains: Legume lectin domain
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, GAL
Carbohydrate polymer
2 bound ligands:
Ligand MN 1 x MN
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: I4122
Unit cell:
a: 122.64Å b: 122.64Å c: 90.56Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.19 0.23

Quick links

Citations

4 review citations

N-linked oligosaccharides as outfitters for glycoprotein folding, form and function.
Mitra et al. (2006)
3 more

2 mentions without citation

Energetics of galactose- and glucose-aromatic amino acid interactions: implications for binding in galactose-specific proteins.
Sujatha et al. (2004)
1 more