› 1g9z

X-ray diffraction
1.8Å resolution

LAGLIDADG HOMING ENDONUCLEASE I-CREI / DNA PRODUCT COMPLEX WITH MAGNESIUM

Released:
DOI: 10.2210/pdb1g9z/pdb
PDB entry 1g9z coloured by chain, front view.
PDB entry 1g9z coloured by chain, side view.
PDB entry 1g9z coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Primary publication:
The homing endonuclease I-CreI uses three metals, one of which is shared between the two active sites.
Chevalier BS, Monnat RJ, Stoddard BL
Nat Struct Biol 8 312-6 (2001)
PMID: 11276249

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113859 (preferred)
Entry contents:
1 distinct polypeptide molecule
4 distinct DNA molecules
Macromolecules (5 distinct):
DNA endonuclease I-CreI Chains: A, B
Molecule details ›
Chains: A, B
Length: 152 amino acids
Theoretical weight: 17.52 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P05725 (Residues: 2-153; Coverage: 93%)
Sequence domains: LAGLIDADG endonuclease
Structure domains: Homing endonucleases
5'-D(*GP*CP*AP*AP*AP*AP*CP*GP*TP*CP*GP*TP*GP*A)-3' Chain: C
Molecule details ›
Chain: C
Length: 14 nucleotides
Theoretical weight: 4.31 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Not provided
5'-D(P*GP*AP*CP*AP*GP*TP*TP*TP*CP*G)-3' Chain: D
Molecule details ›
Chain: D
Length: 10 nucleotides
Theoretical weight: 3.06 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Not provided
5'-D(*CP*GP*AP*AP*AP*CP*TP*GP*TP*CP*TP*CP*AP*C)-3' Chain: E
Molecule details ›
Chain: E
Length: 14 nucleotides
Theoretical weight: 4.22 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Not provided
5'-D(P*GP*AP*CP*GP*TP*TP*TP*TP*GP*C)-3' Chain: F
Molecule details ›
Chain: F
Length: 10 nucleotides
Theoretical weight: 3.05 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21
Unit cell:
a: 43Å b: 67.9Å c: 88.3Å
α: 90° β: 91.6° γ: 90°
R-values:
R R work R free
0.204 0.204 0.249
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

13 review citations

Homing endonucleases: structural and functional insight into the catalysts of intron/intein mobility.
Chevalier et al. (2001)
12 more

12 mentions without citation

DNA conformations and their sequence preferences.
Svozil et al. (2008)
11 more