› 1ga8

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF GALACOSYLTRANSFERASE LGTC IN COMPLEX WITH DONOR AND ACCEPTOR SUGAR ANALOGS.

Released:
DOI: 10.2210/pdb1ga8/pdb
PDB entry 1ga8 coloured by chain, front view.
PDB entry 1ga8 coloured by chain, side view.
PDB entry 1ga8 coloured by chain, top view.
Source organism: Neisseria meningitidis
Primary publication:
Crystal structure of the retaining galactosyltransferase LgtC from Neisseria meningitidis in complex with donor and acceptor sugar analogs.
Persson K, Ly HD, Dieckelmann M, Wakarchuk WW, Withers SG, Strynadka NC
Nat Struct Biol 8 166-75 (2001)
PMID: 11175908

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-galactose + [lipopolysaccharide] = UDP + 3-alpha-D-galactosyl-[lipopolysaccharide]
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188049 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
LgtC Chain: A
Molecule details ›
Chain: A
Length: 311 amino acids
Theoretical weight: 36.11 KDa
Source organism: Neisseria meningitidis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q93EK7 (Residues: 1-311; Coverage: 100%)
Gene name: lgtC
Sequence domains: Glycosyl transferase family 8
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, Z9D
Carbohydrate polymer
2 bound ligands:
Ligand MN 1 x MN
Ligand UPF 1 x UPF
1 modified residue:
Ligand MSE 7 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 39.975Å b: 76.158Å c: 87.228Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.189 0.226
Expression system: Escherichia coli BL21

Quick links

Citations

34 review citations

Lipopolysaccharide endotoxins.
Raetz et al. (2002)
33 more

14 mentions without citation

Calnexin cycle - structural features of the ER chaperone system.
Kozlov et al. (2020)
13 more