› 1gal

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF GLUCOSE OXIDASE FROM ASPERGILLUS NIGER: REFINED AT 2.3 ANGSTROMS RESOLUTION

Released:
DOI: 10.2210/pdb1gal/pdb
PDB entry 1gal coloured by chain, front view.
PDB entry 1gal coloured by chain, side view.
PDB entry 1gal coloured by chain, top view.
Source organism: Aspergillus niger
Primary publication:
Crystal structure of glucose oxidase from Aspergillus niger refined at 2.3 A resolution.
Hecht HJ, Kalisz HM, Hendle J, Schmid RD, Schomburg D
J Mol Biol 229 153-72 (1993)
PMID: 8421298

Function and Biology Details

Reaction catalysed: 
Beta-D-glucose + O(2) = D-glucono-1,5-lactone + H(2)O(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Glucose oxidase Chain: A
Molecule details ›
Chain: A
Length: 583 amino acids
Theoretical weight: 63.33 KDa
Source organism: Aspergillus niger
Expression system: Not provided
UniProt:
  • Canonical: P13006 (Residues: 23-605; Coverage: 100%)
Gene name: gox
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
1 bound ligand:
Ligand NAG 4 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P3121
Unit cell:
a: 66.5Å b: 66.5Å c: 214.5Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.181 0.181 not available
Expression system: Not provided

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Citations

21 review citations

Review: Carbon nanotube based electrochemical sensors for biomolecules.
Jacobs et al. (2010)
20 more

25 mentions without citation

Direct enzymatic bioelectrocatalysis: differentiating between myth and reality.
Milton et al. (2017)
24 more