› 1gfl

X-ray diffraction
1.9Å resolution

STRUCTURE OF GREEN FLUORESCENT PROTEIN

Released:
DOI: 10.2210/pdb1gfl/pdb
PDB entry 1gfl coloured by chain, front view.
PDB entry 1gfl coloured by chain, side view.
PDB entry 1gfl coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
The molecular structure of green fluorescent protein.
Yang F, Moss LG, Phillips GN
Nat Biotechnol 14 1246-51 (1996)
PMID: 9631087

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154559 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 238 amino acids
Theoretical weight: 26.89 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 2-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P41212
Unit cell:
a: 89.226Å b: 89.226Å c: 119.771Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.214 0.262
Expression system: Escherichia coli

Quick links

Citations

104 review citations

The green fluorescent protein.
Tsien RY. (1998)
103 more

126 mentions without citation

Principles Governing the Phase Separation of Multidomain Proteins.
Mohanty et al. (2022)
125 more