› 1gl9

X-ray diffraction
3.2Å resolution

Archaeoglobus fulgidus reverse gyrase complexed with ADPNP

Released:
DOI: 10.2210/pdb1gl9/pdb
PDB entry 1gl9 coloured by chain, front view.
PDB entry 1gl9 coloured by chain, side view.
PDB entry 1gl9 coloured by chain, top view.
Source organism: Archaeoglobus fulgidus DSM 4304
Primary publication:
Crystal structure of reverse gyrase: insights into the positive supercoiling of DNA.
Rodríguez AC, Stock D
EMBO J 21 418-26 (2002)
PMID: 11823434

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128105 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Reverse gyrase Chains: B, C
Molecule details ›
Chains: B, C
Length: 1054 amino acids
Theoretical weight: 121.54 KDa
Source organism: Archaeoglobus fulgidus DSM 4304
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O29238 (Residues: 1-1054; Coverage: 100%)
Gene names: AF_1024, rgy
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ANP 2 x ANP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 132.41Å b: 68.69Å c: 134Å
α: 90° β: 99.7° γ: 90°
R-values:
R R work R free
0.256 0.256 0.332
Expression system: Escherichia coli BL21(DE3)

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Citations

23 review citations

RecQ helicases: multifunctional genome caretakers.
Chu et al. (2009)
22 more

3 mentions without citation

Sequence analysis of bacteriophage T4 DNA packaging/terminase genes 16 and 17 reveals a common ATPase center in the large subunit of viral terminases.
Mitchell et al. (2002)
2 more