› 1gmi

X-ray diffraction
1.7Å resolution

Structure of the c2 domain from novel protein kinase C epsilon

Released:
DOI: 10.2210/pdb1gmi/pdb
PDB entry 1gmi coloured by chain, front view.
PDB entry 1gmi coloured by chain, side view.
PDB entry 1gmi coloured by chain, top view.
Source organism: Rattus rattus
Primary publication:
Structure of the C2 domain from novel protein kinase Cepsilon. A membrane binding model for Ca(2+)-independent C2 domains.
Ochoa WF, Garcia-Garcia J, Fita I, Corbalan-Garcia S, Verdaguer N, Gomez-Fernandez JC
J Mol Biol 311 837-49 (2001)
PMID: 11518534

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140596 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein kinase C epsilon type Chain: A
Molecule details ›
Chain: A
Length: 136 amino acids
Theoretical weight: 15.2 KDa
Source organism: Rattus rattus
Expression system: Escherichia coli
UniProt:
  • Canonical: P09216 (Residues: 1-136; Coverage: 19%)
Gene names: Pkce, Prkce
Sequence domains: C2 domain
Structure domains: C2 domain

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 40.3Å b: 56.5Å c: 60.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.234 0.262
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Structural basis of protein kinase C isoform function.
Steinberg SF. (2008)
10 more

10 mentions without citation

Munc13 C2B domain is an activity-dependent Ca2+ regulator of synaptic exocytosis.
Shin et al. (2010)
9 more