› 1go3

X-ray diffraction
1.75Å resolution

Structure of an archeal homolog of the eukaryotic RNA polymerase II RPB4/RPB7 complex

Released:
DOI: 10.2210/pdb1go3/pdb
PDB entry 1go3 coloured by chain, front view.
PDB entry 1go3 coloured by chain, side view.
PDB entry 1go3 coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Primary publication:
Structure of an archaeal homolog of the eukaryotic RNA polymerase II RPB4/RPB7 complex.
Todone F, Brick P, Werner F, Weinzierl RO, Onesti S
Mol Cell 8 1137-43 (2001)
PMID: 11741548

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176487 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA-directed RNA polymerase subunit Rpo7 Chains: E, M
Molecule details ›
Chains: E, M
Length: 187 amino acids
Theoretical weight: 21.25 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q57840 (Residues: 1-187; Coverage: 100%)
Gene names: MJ0397, rpo7, rpoE, rpoE1
Sequence domains:
Structure domains:
DNA-directed RNA polymerase subunit Rpo4 Chains: F, N
Molecule details ›
Chains: F, N
Length: 107 amino acids
Theoretical weight: 12.34 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q60351 (Residues: 9-115; Coverage: 93%)
Gene names: MJ0039, rpo4, rpoF
Sequence domains: RNA polymerase Rpb4
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P43
Unit cell:
a: 92.391Å b: 92.391Å c: 91.109Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.217 0.238
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

The RNA polymerase II machinery: structure illuminates function.
Woychik et al. (2002)
12 more

17 mentions without citation

A starting point for fluorescence-based single-molecule measurements in biomolecular research.
Gust et al. (2014)
16 more