› 1goy

X-ray diffraction
2Å resolution

HYDROLASE(ENDORIBONUCLEASE)RIBONUCLEASE BI(G SPECIFIC ENDONUCLEASE) (E.C.3.1.27.-) COMPLEXED WITH GUANOSINE-3'-PHOSPHATE (3'-GMP)

Released:
DOI: 10.2210/pdb1goy/pdb
PDB entry 1goy coloured by chain, front view.
PDB entry 1goy coloured by chain, side view.
PDB entry 1goy coloured by chain, top view.
Source organism: Bacillus intermedius
Primary publication:
The structure of substrate-free microbial ribonuclease binase and of its complexes with 3'GMP and sulfate ions.
Polyakov KM, Lebedev AA, Okorokov AL, Panov KI, Schulga AA, Pavlovsky AG, Karpeisky MY, Dodson GG
Acta Crystallogr D Biol Crystallogr 58 744-50 (2002)
PMID: 11976484

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132807 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease Chains: A, B
Molecule details ›
Chains: A, B
Length: 116 amino acids
Theoretical weight: 12.97 KDa
Source organism: Bacillus intermedius
UniProt:
  • Canonical: P00649 (Residues: 48-162; Coverage: 87%)
Sequence domains: ribonuclease
Structure domains: Microbial ribonucleases

Ligands and Environments

2 bound ligands:
Ligand 3GP 1 x 3GP
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 111.38Å b: 69.56Å c: 33.46Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.184 not available

Quick links

Citations

2 review citations

Ribonucleases of different origins with a wide spectrum of medicinal applications.
Fang et al. (2011)
1 more

2 mentions without citation

Designing coarse grained-and atom based-potentials for protein-protein docking.
Tobi D. (2010)
1 more