› 1gp9

X-ray diffraction
2.5Å resolution

A New Crystal Form of the Nk1 Splice Variant of Hgf/Sf Demonstrates Extensive Hinge Movement and Suggests that the Nk1 Dimer Originates by Domain Swapping

Released:
DOI: 10.2210/pdb1gp9/pdb
PDB entry 1gp9 coloured by chain, front view.
PDB entry 1gp9 coloured by chain, side view.
PDB entry 1gp9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A new crystal form of the NK1 splice variant of HGF/SF demonstrates extensive hinge movement and suggests that the NK1 dimer originates by domain swapping.
Watanabe K, Chirgadze DY, Lietha D, de Jonge H, Blundell TL, Gherardi E
J Mol Biol 319 283-8 (2002)
PMID: 12051906

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146878 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hepatocyte growth factor alpha chain Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 177 amino acids
Theoretical weight: 20.33 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P14210 (Residues: 39-210; Coverage: 25%)
Gene names: HGF, HPTA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand EPE 5 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX7.2
Spacegroup: C2221
Unit cell:
a: 73.48Å b: 78.26Å c: 304.46Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.237 0.288
Expression system: Komagataella pastoris

Quick links

Citations

1 review citation

Structural basis of MET receptor dimerization by the bacterial invasion protein InlB and the HGF/SF splice variant NK1.
Niemann HH. (2013)
9 other articles

4 mentions without citation

State of the structure address on MET receptor activation by HGF.
Linossi et al. (2021)
3 more