› 1gt7

X-ray diffraction
2.7Å resolution

L-rhamnulose-1-phosphate aldolase from Escherichia coli

Released:
DOI: 10.2210/pdb1gt7/pdb
PDB entry 1gt7 coloured by chain, front view.
PDB entry 1gt7 coloured by chain, side view.
PDB entry 1gt7 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The structure of L-rhamnulose-1-phosphate aldolase (class II) solved by low-resolution SIR phasing and 20-fold NCS averaging.
Kroemer M, Schulz GE
Acta Crystallogr D Biol Crystallogr 58 824-32 (2002)
PMID: 11976494

Function and Biology Details

Reaction catalysed: 
L-rhamnulose 1-phosphate = glycerone phosphate + (S)-lactaldehyde
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152218 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rhamnulose-1-phosphate aldolase Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T
Length: 274 amino acids
Theoretical weight: 30.17 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P32169 (Residues: 1-274; Coverage: 100%)
Gene names: JW3873, b3902, rhaD, rhuA
Sequence domains: Class II Aldolase and Adducin N-terminal domain
Structure domains: Class II aldolase/adducin N-terminal domain

Ligands and Environments

2 bound ligands:
Ligand ZN 20 x ZN
Ligand PGH 20 x PGH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P3221
Unit cell:
a: 225.76Å b: 225.76Å c: 285.645Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.233 0.233 0.235
Expression system: Escherichia coli

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Citations

1 review citation

A survey of oxidative paracatalytic reactions catalyzed by enzymes that generate carbanionic intermediates: implications for ROS production, cancer etiology, and neurodegenerative diseases.
Bunik et al. (2011)
1 other article

6 mentions without citation

Annotation in three dimensions. PINTS: Patterns in Non-homologous Tertiary Structures.
Stark et al. (2003)
5 more