› 1gtk

X-ray diffraction
1.66Å resolution

Time-resolved and static-ensemble structural chemistry of hydroxymethylbilane synthase

Released:
DOI: 10.2210/pdb1gtk/pdb
PDB entry 1gtk coloured by chain, front view.
PDB entry 1gtk coloured by chain, side view.
PDB entry 1gtk coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Time-resolved and static-ensemble structural chemistry of hydroxymethylbilane synthase.
Helliwell JR, Nieh YP, Habash J, Faulder PF, Raftery J, Cianci M, Wulff M, Hädener A
Faraday Discuss 122 131-44; discussion 171-90 (2003)
PMID: 12555854

Function and Biology Details

Reaction catalysed: 
4 porphobilinogen + H(2)O = hydroxymethylbilane + 4 NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139322 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Porphobilinogen deaminase Chain: A
Molecule details ›
Chain: A
Length: 313 amino acids
Theoretical weight: 33.89 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P06983 (Residues: 1-313; Coverage: 100%)
Gene names: JW5932, b3805, hemC, popE
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand DPM 1 x DPM
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID09
Spacegroup: P21212
Unit cell:
a: 87.5Å b: 75.9Å c: 50.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.198 0.247
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Biosynthesis of heme in mammals.
Ajioka et al. (2006)
1 more

8 mentions without citation

Acute Intermittent Porphyria: An Overview of Therapy Developments and Future Perspectives Focusing on Stabilisation of HMBS and Proteostasis Regulators.
Bustad et al. (2021)
7 more