› 1gzg

X-ray diffraction
1.66Å resolution

Complex of a Mg2-dependent porphobilinogen synthase from Pseudomonas aeruginosa (mutant D139N) with 5-fluorolevulinic acid

Released:
DOI: 10.2210/pdb1gzg/pdb
PDB entry 1gzg coloured by chain, front view.
PDB entry 1gzg coloured by chain, side view.
PDB entry 1gzg coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structure of porphobilinogen synthase from Pseudomonas aeruginosa in complex with 5-fluorolevulinic acid suggests a double Schiff base mechanism.
Frère F, Schubert WD, Stauffer F, Frankenberg N, Neier R, Jahn D, Heinz DW
J Mol Biol 320 237-47 (2002)
PMID: 12079382

Function and Biology Details

Reaction catalysed: 
2 5-aminolevulinate = porphobilinogen + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176810 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Delta-aminolevulinic acid dehydratase Chains: A, B
Molecule details ›
Chains: A, B
Length: 337 amino acids
Theoretical weight: 37.06 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q59643 (Residues: 1-337; Coverage: 100%)
Gene names: PA5243, hemB
Sequence domains: Delta-aminolevulinic acid dehydratase
Structure domains: Aldolase class I

Ligands and Environments

5 bound ligands:
Ligand LAF 4 x LAF
Ligand MG 2 x MG
Ligand NA 2 x NA
Ligand SO4 2 x SO4
Ligand K 2 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P4212
Unit cell:
a: 127.131Å b: 127.131Å c: 86.145Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.175 0.198
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

The biochemistry of heme biosynthesis.
Heinemann et al. (2008)
3 more

11 mentions without citation

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Lawrence et al. (2008)
10 more