› 1gzj

X-ray diffraction
1.62Å resolution

Structure of Thermoascus aurantiacus family 5 endoglucanase

Released:
DOI: 10.2210/pdb1gzj/pdb
PDB entry 1gzj coloured by chain, front view.
PDB entry 1gzj coloured by chain, side view.
PDB entry 1gzj coloured by chain, top view.
Source organism: Thermoascus aurantiacus
Primary publication:
The 1.62 A structure of Thermoascus aurantiacus endoglucanase: completing the structural picture of subfamilies in glycoside hydrolase family 5.
Lo Leggio L, Larsen S
FEBS Lett 523 103-8 (2002)
PMID: 12123813

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186270 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cellulase Chains: A, B
Molecule details ›
Chains: A, B
Length: 304 amino acids
Theoretical weight: 33.66 KDa
Source organism: Thermoascus aurantiacus
UniProt:
  • Canonical: Q8TG26 (Residues: 32-335; Coverage: 96%)
Gene name: eg1
Sequence domains: Cellulase (glycosyl hydrolase family 5)
Structure domains: Glycosidases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P212121
Unit cell:
a: 76.51Å b: 85.85Å c: 89.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.159 0.177

Quick links

Citations

2 review citations

Recent developments on cellulases and carbohydrate-binding modules with cellulose affinity.
Hildén et al. (2004)
1 more

9 mentions without citation

Genomic insights into the fungal lignocellulolytic system of Myceliophthora thermophila.
Karnaouri et al. (2014)
8 more