› 1h1w

X-ray diffraction
2Å resolution

High resolution crystal structure of the human PDK1 catalytic domain

Released:
DOI: 10.2210/pdb1h1w/pdb
PDB entry 1h1w coloured by chain, front view.
PDB entry 1h1w coloured by chain, side view.
PDB entry 1h1w coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
High resolution crystal structure of the human PDK1 catalytic domain defines the regulatory phosphopeptide docking site.
Biondi RM, Komander D, Thomas CC, Lizcano JM, Deak M, Alessi DR, van Aalten DM
EMBO J 21 4219-28 (2002)
PMID: 12169624

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127515 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-phosphoinositide-dependent protein kinase 1 Chain: A
Molecule details ›
Chain: A
Length: 289 amino acids
Theoretical weight: 33.45 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O15530 (Residues: 71-359; Coverage: 52%)
Gene names: PDK1, PDPK1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand GOL 8 x GOL
Ligand SO4 5 x SO4
Ligand ATP 1 x ATP
1 modified residue:
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P3221
Unit cell:
a: 123.013Å b: 123.013Å c: 47.624Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.196 0.223
Expression system: Spodoptera frugiperda

Quick links

Citations

25 review citations

The nuts and bolts of AGC protein kinases.
Pearce et al. (2010)
24 more

21 mentions without citation

Substrate and docking interactions in serine/threonine protein kinases.
Goldsmith et al. (2007)
20 more