› 1h38

X-ray diffraction
2.9Å resolution

Structure of a T7 RNA polymerase elongation complex at 2.9A resolution

Released:
DOI: 10.2210/pdb1h38/pdb
PDB entry 1h38 coloured by chain, front view.
PDB entry 1h38 coloured by chain, side view.
PDB entry 1h38 coloured by chain, top view.
Source organism: Escherichia phage T7
Primary publication:
Structure of a T7 RNA polymerase elongation complex at 2.9 A resolution.
Tahirov TH, Temiakov D, Anikin M, Patlan V, McAllister WT, Vassylyev DG, Yokoyama S
Nature 420 43-50 (2002)
PMID: 12422209

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113883 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
T7 RNA polymerase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 883 amino acids
Theoretical weight: 98.98 KDa
Source organism: Escherichia phage T7
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P00573 (Residues: 1-883; Coverage: 100%)
Gene name: 1
Sequence domains:
Structure domains:
5'-D(*GP*GP*GP*AP*AP*TP*CP*GP*AP*CP *AP*TP*CP*GP*CP*CP*GP*C)-3' Chains: E, H, K, N
Molecule details ›
Chains: E, H, K, N
Length: 18 nucleotides
Theoretical weight: 5.53 KDa
Source organism: Escherichia phage T7
Expression system: Not provided
5'-D(*GP*TP*CP*GP*AP*TP*TP*CP*CP*CP)-3' Chains: G, J, M, P
Molecule details ›
Chains: G, J, M, P
Length: 10 nucleotides
Theoretical weight: 3 KDa
Source organism: Escherichia phage T7
Expression system: Not provided
5'-R(*AP*AP*CP*UP*GP*CP*GP*GP*CP*GP *AP*U)-3' Chains: F, I, L, O
Molecule details ›
Chains: F, I, L, O
Length: 12 nucleotides
Theoretical weight: 3.85 KDa
Source organism: Escherichia phage T7
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL45XU
Spacegroup: P1
Unit cell:
a: 79.907Å b: 84.971Å c: 202.003Å
α: 90.36° β: 92.97° γ: 109.94°
R-values:
R R work R free
0.236 0.236 0.284
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

23 review citations

The many faces of the helix-turn-helix domain: transcription regulation and beyond.
Aravind et al. (2005)
22 more

11 mentions without citation

Modified nucleic acids: replication, evolution, and next-generation therapeutics.
Duffy et al. (2020)
10 more