› 1h6h

X-ray diffraction
1.7Å resolution

Structure of the PX domain from p40phox bound to phosphatidylinositol 3-phosphate

Released:
DOI: 10.2210/pdb1h6h/pdb
PDB entry 1h6h coloured by chain, front view.
PDB entry 1h6h coloured by chain, side view.
PDB entry 1h6h coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The crystal structure of the PX domain from p40(phox) bound to phosphatidylinositol 3-phosphate.
Bravo J, Karathanassis D, Pacold CM, Pacold ME, Ellson CD, Anderson KE, Butler PJ, Lavenir I, Perisic O, Hawkins PT, Stephens L, Williams RL
Mol Cell 8 829-39 (2001)
PMID: 11684018

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172189 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neutrophil cytosol factor 4 Chain: A
Molecule details ›
Chain: A
Length: 143 amino acids
Theoretical weight: 16.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15080 (Residues: 2-144; Coverage: 42%)
Gene names: NCF4, SH3PXD4
Sequence domains: PX domain
Structure domains: Phox-like domain

Ligands and Environments

2 bound ligands:
Ligand PIB 1 x PIB
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: C2
Unit cell:
a: 96.76Å b: 33.642Å c: 76.43Å
α: 90° β: 132.42° γ: 90°
R-values:
R R work R free
0.196 0.196 0.232
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

52 review citations

Membrane recognition by phospholipid-binding domains.
Lemmon MA. (2008)
51 more

35 mentions without citation

Activation and assembly of the NADPH oxidase: a structural perspective.
Groemping et al. (2005)
34 more