› 1h7m

X-ray diffraction
1.96Å resolution

Ribosomal Protein L30e from Thermococcus celer

Released:
DOI: 10.2210/pdb1h7m/pdb
PDB entry 1h7m coloured by chain, front view.
PDB entry 1h7m coloured by chain, side view.
PDB entry 1h7m coloured by chain, top view.
Source organism: Thermococcus celer
Primary publication:
Crystal structure of ribosomal protein L30e from the extreme thermophile Thermococcus celer: thermal stability and RNA binding.
Chen YW, Bycroft M, Wong KB
Biochemistry 42 2857-65 (2003)
PMID: 12627951

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151359 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Large ribosomal subunit protein eL30 Chain: A
Molecule details ›
Chain: A
Length: 102 amino acids
Theoretical weight: 10.99 KDa
Source organism: Thermococcus celer
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P29160 (Residues: 2-101; Coverage: 99%)
Gene names: rpl30, rpl30e
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Structure domains: 60s Ribosomal Protein L30; Chain: A;

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P61
Unit cell:
a: 48.32Å b: 48.32Å c: 86.42Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.17 0.165 0.225
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 citation in other articles

Genome-wide patterns of nucleotide substitution reveal stringent functional constraints on the protein sequences of thermophiles.
Friedman et al. (2004)
10 more

6 mentions without citation

Reviewing Challenges of Predicting Protein Melting Temperature Change Upon Mutation Through the Full Analysis of a Highly Detailed Dataset with High-Resolution Structures.
Louis et al. (2021)
5 more