› 1h8o

X-ray diffraction
2.75Å resolution

Three-dimensional structure of anti-ampicillin single chain Fv fragment.

Released:
DOI: 10.2210/pdb1h8o/pdb
PDB entry 1h8o coloured by chain, front view.
PDB entry 1h8o coloured by chain, side view.
PDB entry 1h8o coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Selection, characterization and x-ray structure of anti-ampicillin single-chain Fv fragments from phage-displayed murine antibody libraries.
Burmester J, Spinelli S, Pugliese L, Krebber A, Honegger A, Jung S, Schimmele B, Cambillau C, Plückthun A
J Mol Biol 309 671-85 (2001)
PMID: 11397088

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213013 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
MUTANT AL2 6E7P9G Chains: A, B
Molecule details ›
Chains: A, B
Length: 252 amino acids
Theoretical weight: 27.01 KDa
Source organism: Mus musculus
Expression system: Escherichia coli K-12
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 59.69Å b: 89.85Å c: 97.26Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.178 0.258
Expression system: Escherichia coli K-12

Quick links

Citations

20 citation in other articles

The POM monoclonals: a comprehensive set of antibodies to non-overlapping prion protein epitopes.
Polymenidou et al. (2008)
19 more