› 1h8s

X-ray diffraction
2.4Å resolution

Three-dimensional structure of anti-ampicillin single chain Fv fragment complexed with the hapten.

Released:
DOI: 10.2210/pdb1h8s/pdb
PDB entry 1h8s coloured by chain, front view.
PDB entry 1h8s coloured by chain, side view.
PDB entry 1h8s coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Selection, characterization and x-ray structure of anti-ampicillin single-chain Fv fragments from phage-displayed murine antibody libraries.
Burmester J, Spinelli S, Pugliese L, Krebber A, Honegger A, Jung S, Schimmele B, Cambillau C, Plückthun A
J Mol Biol 309 671-85 (2001)
PMID: 11397088

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213014 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
MUTANT AL2 6E7P9G Chains: A, B
Molecule details ›
Chains: A, B
Length: 252 amino acids
Theoretical weight: 27.01 KDa
Source organism: Mus musculus
Expression system: Escherichia coli K-12
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand AIC 1 x AIC
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 58.93Å b: 89.27Å c: 94.41Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.204 0.273
Expression system: Escherichia coli K-12

Quick links

Citations

19 citation in other articles

The POM monoclonals: a comprehensive set of antibodies to non-overlapping prion protein epitopes.
Polymenidou et al. (2008)
18 more

1 mention without citation

An anti-hapten camelid antibody reveals a cryptic binding site with significant energetic contributions from a nonhypervariable loop.
Fanning et al. (2011)