› 1hb0

X-ray diffraction
2.05Å resolution

Snapshots of serine protease catalysis: (D) acyl-enzyme intermediate between porcine pancreatic elastase and human beta-casomorphin-7 jumped to pH 10 for 2 minutes

Released:
DOI: 10.2210/pdb1hb0/pdb
PDB entry 1hb0 coloured by chain, front view.
PDB entry 1hb0 coloured by chain, side view.
PDB entry 1hb0 coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
X-ray snapshots of serine protease catalysis reveal a tetrahedral intermediate.
Wilmouth RC, Edman K, Neutze R, Wright PA, Clifton IJ, Schneider TR, Schofield CJ, Hajdu J
Nat Struct Biol 8 689-94 (2001)
PMID: 11473259

Function and Biology Details

Reaction catalysed: 
Hydrolysis of proteins, including elastin. Preferential cleavage: Ala-|-.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133451 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chymotrypsin-like elastase family member 1 Chain: B
Molecule details ›
Chain: B
Length: 240 amino acids
Theoretical weight: 26.01 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P00772 (Residues: 27-266; Coverage: 96%)
Gene names: CELA1, ELA1
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P212121
Unit cell:
a: 49.63Å b: 57.6Å c: 73.44Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.199 0.246

Quick links

Citations

7 review citations

The protein structures that shape caspase activity, specificity, activation and inhibition.
Fuentes-Prior et al. (2004)
6 more

1 mention without citation

Crystallization and preliminary X-ray diffraction analyses of several forms of the CfaB major subunit of enterotoxigenic Escherichia coli CFA/I fimbriae.
Li et al. (2009)